Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.
Identifieur interne : 000B78 ( Main/Exploration ); précédent : 000B77; suivant : 000B79Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.
Auteurs : Yolanda T. Chong [Canada] ; Judice L Y. Koh [Canada] ; Helena Friesen [Canada] ; Supipi Kaluarachchi Duffy ; Kaluarachchi Duffy [Canada] ; Michael J. Cox [Canada] ; Alan Moses [Canada] ; Jason Moffat [Canada] ; Charles Boone [Canada] ; Brenda J. Andrews [Canada]Source :
- Cell [ 1097-4172 ] ; 2015.
Descripteurs français
- KwdFr :
- Analyse sur cellule unique (MeSH), Machine à vecteur de support (MeSH), Protéines de Saccharomyces cerevisiae (analyse), Protéines de Saccharomyces cerevisiae (métabolisme), Protéome (analyse), Saccharomyces cerevisiae (composition chimique), Saccharomyces cerevisiae (cytologie), Saccharomyces cerevisiae (métabolisme).
- MESH :
- analyse : Protéines de Saccharomyces cerevisiae, Protéome.
- composition chimique : Saccharomyces cerevisiae.
- cytologie : Saccharomyces cerevisiae.
- métabolisme : Protéines de Saccharomyces cerevisiae, Saccharomyces cerevisiae.
- Analyse sur cellule unique, Machine à vecteur de support.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Proteome, Saccharomyces cerevisiae Proteins.
- chemistry : Saccharomyces cerevisiae.
- cytology : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins.
- Single-Cell Analysis, Support Vector Machine.
Abstract
Proteomics has proved invaluable in generating large-scale quantitative data; however, the development of systems approaches for examining the proteome in vivo has lagged behind. To evaluate protein abundance and localization on a proteome scale, we exploited the yeast GFP-fusion collection in a pipeline combining automated genetics, high-throughput microscopy, and computational feature analysis. We developed an ensemble of binary classifiers to generate localization data from single-cell measurements and constructed maps of ∼3,000 proteins connected to 16 localization classes. To survey proteome dynamics in response to different chemical and genetic stimuli, we measure proteome-wide abundance and localization and identified changes over time. We analyzed >20 million cells to identify dynamic proteins that redistribute among multiple localizations in hydroxyurea, rapamycin, and in an rpd3Δ background. Because our localization and abundance data are quantitative, they provide the opportunity for many types of comparative studies, single cell analyses, modeling, and prediction. VIDEO ABSTRACT.
DOI: 10.1016/j.cell.2015.04.051
PubMed: 26046442
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.</title><author><name sortKey="Chong, Yolanda T" sort="Chong, Yolanda T" uniqKey="Chong Y" first="Yolanda T" last="Chong">Yolanda T. Chong</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Koh, Judice L Y" sort="Koh, Judice L Y" uniqKey="Koh J" first="Judice L Y" last="Koh">Judice L Y. Koh</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Friesen, Helena" sort="Friesen, Helena" uniqKey="Friesen H" first="Helena" last="Friesen">Helena Friesen</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Duffy, Supipi Kaluarachchi" sort="Duffy, Supipi Kaluarachchi" uniqKey="Duffy S" first="Supipi Kaluarachchi" last="Duffy">Supipi Kaluarachchi Duffy</name></author><author><name sortKey="Duffy, Kaluarachchi" sort="Duffy, Kaluarachchi" uniqKey="Duffy K" first="Kaluarachchi" last="Duffy">Kaluarachchi Duffy</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Cox, Michael J" sort="Cox, Michael J" uniqKey="Cox M" first="Michael J" last="Cox">Michael J. Cox</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Moses, Alan" sort="Moses, Alan" uniqKey="Moses A" first="Alan" last="Moses">Alan Moses</name><affiliation wicri:level="4"><nlm:affiliation>Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Moffat, Jason" sort="Moffat, Jason" uniqKey="Moffat J" first="Jason" last="Moffat">Jason Moffat</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Boone, Charles" sort="Boone, Charles" uniqKey="Boone C" first="Charles" last="Boone">Charles Boone</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Andrews, Brenda J" sort="Andrews, Brenda J" uniqKey="Andrews B" first="Brenda J" last="Andrews">Brenda J. Andrews</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada. Electronic address: brenda.andrews@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26046442</idno><idno type="pmid">26046442</idno><idno type="doi">10.1016/j.cell.2015.04.051</idno><idno type="wicri:Area/Main/Corpus">000C44</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000C44</idno><idno type="wicri:Area/Main/Curation">000C44</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000C44</idno><idno type="wicri:Area/Main/Exploration">000C44</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.</title><author><name sortKey="Chong, Yolanda T" sort="Chong, Yolanda T" uniqKey="Chong Y" first="Yolanda T" last="Chong">Yolanda T. Chong</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Koh, Judice L Y" sort="Koh, Judice L Y" uniqKey="Koh J" first="Judice L Y" last="Koh">Judice L Y. Koh</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Friesen, Helena" sort="Friesen, Helena" uniqKey="Friesen H" first="Helena" last="Friesen">Helena Friesen</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Duffy, Supipi Kaluarachchi" sort="Duffy, Supipi Kaluarachchi" uniqKey="Duffy S" first="Supipi Kaluarachchi" last="Duffy">Supipi Kaluarachchi Duffy</name></author><author><name sortKey="Duffy, Kaluarachchi" sort="Duffy, Kaluarachchi" uniqKey="Duffy K" first="Kaluarachchi" last="Duffy">Kaluarachchi Duffy</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Cox, Michael J" sort="Cox, Michael J" uniqKey="Cox M" first="Michael J" last="Cox">Michael J. Cox</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Moses, Alan" sort="Moses, Alan" uniqKey="Moses A" first="Alan" last="Moses">Alan Moses</name><affiliation wicri:level="4"><nlm:affiliation>Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Moffat, Jason" sort="Moffat, Jason" uniqKey="Moffat J" first="Jason" last="Moffat">Jason Moffat</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Boone, Charles" sort="Boone, Charles" uniqKey="Boone C" first="Charles" last="Boone">Charles Boone</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Andrews, Brenda J" sort="Andrews, Brenda J" uniqKey="Andrews B" first="Brenda J" last="Andrews">Brenda J. Andrews</name><affiliation wicri:level="4"><nlm:affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada. Electronic address: brenda.andrews@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author></analytic><series><title level="j">Cell</title><idno type="eISSN">1097-4172</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Proteome (analysis)</term><term>Saccharomyces cerevisiae (chemistry)</term><term>Saccharomyces cerevisiae (cytology)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Saccharomyces cerevisiae Proteins (analysis)</term><term>Saccharomyces cerevisiae Proteins (metabolism)</term><term>Single-Cell Analysis (MeSH)</term><term>Support Vector Machine (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse sur cellule unique (MeSH)</term><term>Machine à vecteur de support (MeSH)</term><term>Protéines de Saccharomyces cerevisiae (analyse)</term><term>Protéines de Saccharomyces cerevisiae (métabolisme)</term><term>Protéome (analyse)</term><term>Saccharomyces cerevisiae (composition chimique)</term><term>Saccharomyces cerevisiae (cytologie)</term><term>Saccharomyces cerevisiae (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Proteome</term><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines de Saccharomyces cerevisiae</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de Saccharomyces cerevisiae</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Single-Cell Analysis</term><term>Support Vector Machine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse sur cellule unique</term><term>Machine à vecteur de support</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Proteomics has proved invaluable in generating large-scale quantitative data; however, the development of systems approaches for examining the proteome in vivo has lagged behind. To evaluate protein abundance and localization on a proteome scale, we exploited the yeast GFP-fusion collection in a pipeline combining automated genetics, high-throughput microscopy, and computational feature analysis. We developed an ensemble of binary classifiers to generate localization data from single-cell measurements and constructed maps of ∼3,000 proteins connected to 16 localization classes. To survey proteome dynamics in response to different chemical and genetic stimuli, we measure proteome-wide abundance and localization and identified changes over time. We analyzed >20 million cells to identify dynamic proteins that redistribute among multiple localizations in hydroxyurea, rapamycin, and in an rpd3Δ background. Because our localization and abundance data are quantitative, they provide the opportunity for many types of comparative studies, single cell analyses, modeling, and prediction. VIDEO ABSTRACT.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26046442</PMID><DateCompleted><Year>2015</Year><Month>08</Month><Day>17</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1097-4172</ISSN><JournalIssue CitedMedium="Internet"><Volume>161</Volume><Issue>6</Issue><PubDate><Year>2015</Year><Month>Jun</Month><Day>04</Day></PubDate></JournalIssue><Title>Cell</Title><ISOAbbreviation>Cell</ISOAbbreviation></Journal><ArticleTitle>Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.</ArticleTitle><Pagination><MedlinePgn>1413-24</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cell.2015.04.051</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0092-8674(15)00526-7</ELocationID><Abstract><AbstractText>Proteomics has proved invaluable in generating large-scale quantitative data; however, the development of systems approaches for examining the proteome in vivo has lagged behind. To evaluate protein abundance and localization on a proteome scale, we exploited the yeast GFP-fusion collection in a pipeline combining automated genetics, high-throughput microscopy, and computational feature analysis. We developed an ensemble of binary classifiers to generate localization data from single-cell measurements and constructed maps of ∼3,000 proteins connected to 16 localization classes. To survey proteome dynamics in response to different chemical and genetic stimuli, we measure proteome-wide abundance and localization and identified changes over time. We analyzed >20 million cells to identify dynamic proteins that redistribute among multiple localizations in hydroxyurea, rapamycin, and in an rpd3Δ background. Because our localization and abundance data are quantitative, they provide the opportunity for many types of comparative studies, single cell analyses, modeling, and prediction. VIDEO ABSTRACT.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chong</LastName><ForeName>Yolanda T</ForeName><Initials>YT</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koh</LastName><ForeName>Judice L Y</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Friesen</LastName><ForeName>Helena</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duffy</LastName><ForeName>Supipi Kaluarachchi</ForeName><Initials>SK</Initials></Author><Author ValidYN="N"><LastName>Duffy</LastName><ForeName>Kaluarachchi</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cox</LastName><ForeName>Michael J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moses</LastName><ForeName>Alan</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moffat</LastName><ForeName>Jason</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boone</LastName><ForeName>Charles</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andrews</LastName><ForeName>Brenda J</ForeName><Initials>BJ</Initials><AffiliationInfo><Affiliation>The Donnelly Centre, University of Toronto, Toronto, ON M5S3E1, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S3E1, Canada. Electronic address: brenda.andrews@utoronto.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>MOP-97939</GrantID><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Cell</MedlineTA><NlmUniqueID>0413066</NlmUniqueID><ISSNLinking>0092-8674</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Cell. 2015 Jul 2;162(1):221</RefSource><Note>Duffy, Kaluarachchi [corrected to Duffy, Supipi Kaluarachchi]</Note></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059010" MajorTopicYN="N">Single-Cell Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060388" MajorTopicYN="Y">Support Vector Machine</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>02</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>04</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26046442</ArticleId><ArticleId IdType="pii">S0092-8674(15)00526-7</ArticleId><ArticleId IdType="doi">10.1016/j.cell.2015.04.051</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><noCountry><name sortKey="Duffy, Supipi Kaluarachchi" sort="Duffy, Supipi Kaluarachchi" uniqKey="Duffy S" first="Supipi Kaluarachchi" last="Duffy">Supipi Kaluarachchi Duffy</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Chong, Yolanda T" sort="Chong, Yolanda T" uniqKey="Chong Y" first="Yolanda T" last="Chong">Yolanda T. Chong</name></region><name sortKey="Andrews, Brenda J" sort="Andrews, Brenda J" uniqKey="Andrews B" first="Brenda J" last="Andrews">Brenda J. Andrews</name><name sortKey="Boone, Charles" sort="Boone, Charles" uniqKey="Boone C" first="Charles" last="Boone">Charles Boone</name><name sortKey="Cox, Michael J" sort="Cox, Michael J" uniqKey="Cox M" first="Michael J" last="Cox">Michael J. Cox</name><name sortKey="Duffy, Kaluarachchi" sort="Duffy, Kaluarachchi" uniqKey="Duffy K" first="Kaluarachchi" last="Duffy">Kaluarachchi Duffy</name><name sortKey="Friesen, Helena" sort="Friesen, Helena" uniqKey="Friesen H" first="Helena" last="Friesen">Helena Friesen</name><name sortKey="Koh, Judice L Y" sort="Koh, Judice L Y" uniqKey="Koh J" first="Judice L Y" last="Koh">Judice L Y. Koh</name><name sortKey="Moffat, Jason" sort="Moffat, Jason" uniqKey="Moffat J" first="Jason" last="Moffat">Jason Moffat</name><name sortKey="Moses, Alan" sort="Moses, Alan" uniqKey="Moses A" first="Alan" last="Moses">Alan Moses</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B78 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B78 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26046442
|texte= Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26046442" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |